ima mesylate  (Santa Cruz Biotechnology)

Journal: Signal Transduction and Targeted Therapy

Article Title: EPHA2 mediates PDGFA activity and functions together with PDGFRA as prognostic marker and therapeutic target in glioblastoma

doi: 10.1038/s41392-021-00855-2

Figure Lengend Snippet: Co-inhibition of PDGFRA and EPHA2 synergetically inhibits GBM cells. a IC 50 measurement of LN18 cells with forced expression of EPHA2 (left panel) or knockdown of EPHA2 (right panel) through MTT assay. b Antibody array analysis of LN18 cells treated with vehicle, EPHA2 inhibitor (ALW), and PDGFRA inhibitor (IMA). Significant changed proteins are labeled with frame and listed separately. c MTT assay-based drug combination evaluation in four GBM cell lines. d Representative images of orthotopic growth of U251 cells treated with vehicle, IMA, ALW, or IMA + ALW. e Statistic graph of tumor size using bioluminescence signal intensity. n = 8 for each group. f Representative immunohistochemistry images of Ki67 on tissue sections from mice with orthotopic GBM tumors treated by vehicle, IMA, ALW, or IMA + ALW. Scale Bar = 200 μm (upper) and 100 μm (lower).

Article Snippet: To examine the combined effects of Imatinib (IMA) and ALW-II-41-27 (ALW) treatment, mice were treated with vehicle control (i.p.), IMA (25 mg/kg, i.p., Selleckchem, S1026), ALW (10 mg/kg, i.p., MedChemExpress, HY-18007), or the combination of IMA and ALW.

Techniques: Inhibition, Expressing, Knockdown, MTT Assay, Ab Array, Labeling, Immunohistochemistry

Journal: Scientific Reports

Article Title: Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination

doi: 10.1038/s41598-021-91790-3

Figure Lengend Snippet: A two steps PCR recombination assay is used to identify Vk to Jk1 or Vk to Jk2 rearrangements from pre-B cells upon RAG induction with Imatinib or GSK. ( A ) A Sybr Green stained 1.5% Agarose TBE gel in which the recombination PCR reactions templated with initial 1:5 dilutions of genomic DNA extracted from each distinct cell treatment lot (2 × 10 6 cells) are electrophoretically resolved. The cells were either unexposed (gel reaction lanes 4 and 5) or subjected for 48 h to 1 GHz EMF irradiation (lanes 1 to 3 and 6 to 8) with the generator setting at 13 dBm. The color code designating the positions of exposed EMF (exp.Well) wells in the dish is the same with the one used in supplementary Fig. Sb. Last lane (9) of the gel, -DNA control reaction. The bottom black box (cropped from a distinct gel) displays Hisone H1 PCR reactions templated with the same amount of genomic DNA as the recombination reactions above(control genomic DNA). ( B ) Identified Vk to Jk2 recombined products were quantified from scanned gels corresponding to PCR reactions from cells +/− Irradiation and the calculated ratios of band intensities expressed + EMF/−EMF(irradiated/nonexposed) for each well (color code consistent with that shown in Fig. S). The histograms represent the average values of three independent quantified experiments. EMF-Electromagnetic Field, Recombination pharmacological stimuli (Imatinib, IMA) versus (GSK-690693, GSK). H1, histone H1 control reaction PCR reactions. Darker font histograms correspond to lower 7 dBm (l) and brighter to higher 13 dBm(h) generator power settings.

Article Snippet: The cells were maintained in RPMI 1640 medium, supplemented with 10% FBS (both from GIBCO), 50 µM 2-mercaptoetanol and induced at 0.5 × 10 6 cells/ml density either with 3 μM Imatinib Mesylate (IMA) (SIGMA-ALDRICH) or with 10 µM GSK-690693(GSK)( GLAXOSMITHKLINE, SELLECK-chem) in solutions with 0.1% DMSO.

Techniques: Recombination Assay, SYBR Green Assay, Staining, Irradiation

Journal: Scientific Reports

Article Title: Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination

doi: 10.1038/s41598-021-91790-3

Figure Lengend Snippet: The EMF power dose-cell recombination response curves at 720, 850, 950, 1000 and 1224 MHz for both types of pharmacological agents stimulating RAG expression ( A ) (GSK-690693, GSK), and ( B ) (Imatinib, IMA). Cell Recomb. Fr. expresses the ratio values of measured Vk-Jk2 recombination quantified from cells grown in + EMF/-EMF (irradiated/non-exposed) conditions. Bottom abscissa displays logarithm of S power flux density values (Power µW/cm 2 ) measured around the emitting antenna inside the CO 2 5 vol%, and 95% water humidity incubator air conditions, expressed as a single range in all panels(logartithmic scale). The black dotted line denote a level of EMF induced two-fold recombination reduction (Cell recomb. Fr. = 0.5), whereas when this level is not reached in the experiment the coordinates of the lowest obtained Cell Recomb. Fr. are given. The red dotted line connecting markers is just a Moving Window Average line which accounts for the average between successive data points displaying the trend of data variation. The error bars represent standard deviation (SD) values from three independent experiments.

Article Snippet: The cells were maintained in RPMI 1640 medium, supplemented with 10% FBS (both from GIBCO), 50 µM 2-mercaptoetanol and induced at 0.5 × 10 6 cells/ml density either with 3 μM Imatinib Mesylate (IMA) (SIGMA-ALDRICH) or with 10 µM GSK-690693(GSK)( GLAXOSMITHKLINE, SELLECK-chem) in solutions with 0.1% DMSO.

Techniques: Expressing, Irradiation, Standard Deviation

Journal: Scientific Reports

Article Title: Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination

doi: 10.1038/s41598-021-91790-3

Figure Lengend Snippet: Variation of cell recombination fraction (+ EMF/-EMF) with the field irradiation frequency shown in each panel for a constant receiver EMF electric intensity field E calculated in the cell culture medium. ( A ) EMF electric field intensity E 0.4 V/m, ( B ) EMF electric field intensity E 0.6 V/m. The pharmacological agents stimulating RAG expression GSK-690693, GSK-blue, and Imatinib, IMA-red. The pale green dotted line shows the relative constant distribution of measured electric field as a function of frequency. The error bars represent standard deviation (SD) values from three independent experiments.

Article Snippet: The cells were maintained in RPMI 1640 medium, supplemented with 10% FBS (both from GIBCO), 50 µM 2-mercaptoetanol and induced at 0.5 × 10 6 cells/ml density either with 3 μM Imatinib Mesylate (IMA) (SIGMA-ALDRICH) or with 10 µM GSK-690693(GSK)( GLAXOSMITHKLINE, SELLECK-chem) in solutions with 0.1% DMSO.

Techniques: Irradiation, Cell Culture, Expressing, Standard Deviation

Journal: Scientific Reports

Article Title: Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination

doi: 10.1038/s41598-021-91790-3

Figure Lengend Snippet: ( A – I ) Immuno-fluorescence detection of γH2AX phosphorylated histone foci in pre-B cells exposed to EMF or X ray irradiation. The γ H2AX foci are shown in bright green—γ-H2AX, whereas DNA staining is in blue – nuclei. ( A ) Control solvent (DMSO) treated cells Nonirradiated (NIR); ( B) Control (DMSO) treated 1 Gy X-ray irradiated cells(X-Ray); ( C ) Control (DMSO)treated EMF exposed (waves at 950 MHz, with emission generator power setting set at 7 dBm-EMF); ( D ) IMA treated NIR; ( E ) IMA, X-ray; ( F ) IMA, EMF; ( G ) GSK, NIR; ( H ) GSK, X-ray; ( I) GSK, EMF. ( J) Number of counted foci per /cell represented as histograms. W1/4 refers to growing plate wells 1 and 4, W2/5 wells 2 and 5 and W3/6 wells 3 and 6 equivalent positions with respect to which cells were EMF irradiated, with generator power setting set at 7 dBm and 13 dBm. GSK is cells treatment with 10 µM GSK-690693, IMA their treatment with 3 µM Imatinib. ANDOR camera assisted by IQ Live Cell Imaging software and foci analysis with Imaris for Cell Biologists software (both from OXFORD Instruments).

Article Snippet: The cells were maintained in RPMI 1640 medium, supplemented with 10% FBS (both from GIBCO), 50 µM 2-mercaptoetanol and induced at 0.5 × 10 6 cells/ml density either with 3 μM Imatinib Mesylate (IMA) (SIGMA-ALDRICH) or with 10 µM GSK-690693(GSK)( GLAXOSMITHKLINE, SELLECK-chem) in solutions with 0.1% DMSO.

Techniques: Fluorescence, Irradiation, Staining, Live Cell Imaging, Software